Abstract

RPLP1 GENE COULD BE A POTENTIAL BIOMARKER OF MELANOMA BRAIN METASTASES (MBM): INSIGHTS FROM SCRNASEQ DATA ANALYSIS

Sumedh Choukidar, Prasad Choukidar, Subodh Choukidar, *Dr. Archana Panche and Dr. Sanjay Harke

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Abstract

Melanoma brain metastases (MBM) is a secondary brain tumor that has metastasized from skin to the brain. Single cell RNA sequence analysis is a next generation sequencing method that is mainly used to analyze differences in genetic and protein information between cells and to better understand the specific roles of the gene. In this research our objective was to analyze the single cell RNA sequence data and identify the markers for the disease. We analyzed the samples of scRNAseq data of Melanoma brain metastases (MBM) using GALAXY server. After performing the several steps of the scRNAseq pipeline we were able to find the possible biomarker for the secondary brain tumor. ‘RPLP1’ gene is found to be promoting tumor metastasis and is highly expressed in all the six samples that are taken for the analysis. ‘RPLP1’ gene could be a potential biomarker of secondary brain tumor metastasis. This study will further lead practitioners and oncologists for the implementation of possible treatments against Melanoma brain metastases (MBM).

Keywords: Scrnaseq, Melanoma brain metastases (MBM), Bioinformatics.