Abstract

THE EFFECTS OF PISTACIA ATLANTICA ON TESTOSTERONEINDUCED BENIGN PROSTATIC HYPERPLASIA IN RATS

Raana Zakeri, *Ali Mirzaei, Mehdi Ghavamizadeh

Abstract

Benign prostatic hyperplasia (BPH) is a chronic urological disorder. The aim of this study was evaluation the effects of of Pistacia Atlantica on testosterone- induced benign prostatic hyperplasia in wistar male rats. Materials and Methods: Hydroalcoholic extract of Pistacia Atlantica was prepared by maceration method. Forty rats were prepared and divided into four groups with 10 animals in each group. BPH induces through daily subcutaneous injection of 3mg testosterone enantat (TE) dissolved in corn oil, for 35 days. Pistacia atlantica extract was administered daily by oral gavage at doses of 250 and 500 mg / kg along with the TE injection for 35 days. The negative control received daily 1ml / kg of corn oil via subcutaneous injection and 1ml / kg normal saline by oral gavage. At the end of the experiment blood was collected by heart puncture under light chloroform anesthesia and finally all rats were sacrificed and prostate gland was removed. serum was collected immediately by centrifuge for of testosterone, PSA(prostate specific antigen) and blood urea concentration. The data are expressed as mean ±SD. and analyzed by one-way ANOVA. Results: Daily administration of 250 and 500mg /kg of pistacia atlantica extract led to marked decreases in the serum PSA and the serum testosterone levels in the treated groups compared to positive group .Insignificant decrease was reported in the blood urea concentrationin the treated groups compared to positive group. Conclusion:Pistacia atlantica extract effectively inhibits the development of BPH induced by testosterone in a rat model.

Keywords: benign prostatic hyperplasia, testosterone, prostate, pistacia atlantica.