Abstract

STUDIES ON NEPHROPROTECTIVE ACTIVITY OF TEPHROSIA PURPURIA LINN ALCOHOLIC EXTRACT BY CISPLATIN INDUCED MODEL USING ALBINO RATS

Parameshappa B* and Dr. VeereshBabu P

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Abstract

Background: In traditional system of medicine Tephrosia purpuria linn plant is extensively used for its activities such as Anti-inflammatory, antiulcer, cardio-protective, anti-emetic, anti-fungal, anti-diarrhoeal, hepatoprotective effects. Further no scientific data is available on the nephroprotective activity of this plant, hence in the present study the nephroprotective activity of this plant was explored in experimental rats model. Objectives: The nephroproctive activity for the alcoholic extracts of Tephrosia purpuria linn whole plant was performed in albino rats by Cisplatin induced nephrotoxicity model. Methods: Alcoholic extract of Tephrosia purpuria linn plant was prepared by using Soxhlet extraction process. The extract was subjected for LD50 studies in mice upto dose level of 2000 mg/kg, p.o. Nephroprotective activity was performed for alcoholic extract of Tephrosia purpuria linn plant by Cisplatin induced nephrotoxicity model. Serum samples were analyzed for biochemical parameters Alkaline phosphate, Blood urea nitrogen, Cholesterol, Creatinine, Albumin and Uric acid using semi auto-analyzer. Results and Discussion: The LD50 studies indicated that none of the mice produced abnormal behavior or mortality rate. Alcoholic extract of Tephrosia purpuria linn showed significant Nephroprotective effect. Bio chemical parameters levels were also significantly altered after treatment with different dose of the alcoholic extract in nephrotoxic rats. Conclusion: Preliminary phytochemical investigation indicated presence of phytoconstituents like flavonoids, saponins, steroidal triterpines, tannins, carbohydrates and glycosides in the alcoholic extract. Tephrosia purpuria linn whole plant alcoholic extract showed significant neproprotective action.

Keywords: Alcoholic extract of Tephrosia purpuria linn, Phytoconstituents Nephroprotective activity, Cisplatin, Bio chemical parameters.