Abstract

THE ASSOCIATION OF HHEX GENE POLYMORPHISM WITH THE INCIDENCE OF POLYCYSTIC OVARY SYNDROME (PCOS) IN IRAQ.

Ismail.A.Abdul-Hassan*, Mohammed T. Al-Tamimi, Saad.S.Al-dujaily

Abstract

This study was conducted to evaluate whether polymorphisms of HHEX gene (rs1111875) responsible for insulin secretion are associated with PCOS and PCOS with type2 diabetes mellitus (T2DM) in Iraqi women. Sixty PCOS and PCOS plus T2DM patients and 30 apparently healthy individuals were used in this study. Blood samples were obtained for DNA analysis and hormonal measurements. Genotyping of the HHEX gene was carried out by using PCR-RFLP method. There were no significant differences in allele frequency of the g.92703125A˃G mutation (rs1111875) between the two groups of Iraqi patients women and controls. The AG heterozygous genotype percentage was significantly higher (p<0.01) in PCOS than control (56.66 versus 39.28% ), and increased PCOS risk (OR= 1.328 , χ² =6.277). Significant association were found among AG and GG genotypes with LH and testosterone levels , in addition to F.B.S and LH : FSH ratio. An association was found between two genotypes of g.92703125A˃G mutation (rs1111875) with the occurrence of both diabetic PCOS and PCOS in the Iraqi women population.

Keywords: HHEX gene, PCOS, T2DM, PCR-RFLP.