Abstract

HEALING ACTIVITY OF LEPIDIUM SATIVUM L. IN EXPERIMENTALLY INDUCED DIABETIC RATS.

Rajkumari Sahane*, Sampatkumar Donthula, Priyanka Nalavde.

Abstract

Aim: The aim of present study was to evaluate the wound healing potential of Lepidium sativum seeds when administered orally as well as topically in STZ induced diabetic rats. Methods: Excision wound model was used in Sprague dawley rats of either sex. For assessing the wound healing property ethanolic extract of seeds was prepared and formulated into 20 and 30 mg/kg solution in water and also as 2% ointment in Vaseline. Control animals were treated with water as vehicle and Vaseline topically. The parameters studied include wound area measurement, % wound healing, Hydroxyproline and Hexosamine content along with some biochemical parameters. Results: Significant wound healing activity was observed both topically and orally treated groups. Ethanolic extract treated groups showed a significant increase in % wound healing, hydroxyproline and hexosamine content, RBC & WBC count and total protein content. Whereas consequently decreased wound area, clotting time, total cholesterol and serum triglycerides levels. For wound healing property higher oral dose (30 mg/kg) was found to be more effective than other and its observations were comparable with those of standard (mupirocin). Conclusion: The data of this study indicates that ethanolic seed extract of Lepidium sativum is effective in treatment of diabetic wounds. This property of the plant may be attributed to its antihyperglycaemic and anti-inflammatory property.

Keywords: Lepidium sativum seeds, excision wound model, hexosamine, hydroxyproline.