Abstract

STUDIES ON THE PROFILE OF HEAT SHOCK PROTEINS IN SELECTED HUMAN PATHOGENIC BACTERIA

Diona Mary and Anandharaj*

Abstract

Heat shock proteins are evolutionarily highly conserved polypeptides that are produced under a variety of stress conditions to preserve cellular functions. In the present study, The bacterial species Pseudomonas aeruginosa, Salmonella typhi, Proteus vulgaris, Staphylococcus aureus, Klebsiella pneumoniae were grown in their respective selective medium for identification of the species. The identified species were grown in nutrient broth for 48 hours at 37°C in a rotary shaker. About 5 ml of each culture was transferred to new and sterile tubes and were incubated at 50°C for an hour (Heat shock).Then both the cell types ie., heat shocked cells and normal cell suspensions were centrifuged at 10, 000 rpm for 15 minutes. The supernatant was loaded in the wells of the SDS-PAGE gel. The protein bands were observed and heat shock proteins were identified by comparing the protein profile of the normal as well as heat shocked cells. The molecular mass of the suspected heat shock proteins were determined by comparing with the molecular mass markers. All the bacterial cells showed an increase in the total cellular protein content after heat shock.

Keywords: Heat shock proteins, Pseudomonas aeruginosa, Salmonella typhi, Proteus vulgaris, Staphylococcus aureus, Klebsiella pneumonia, molecular mass markers.