Abstract

STUDY OF ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY OF PUNICA GRANATUM PEEL AND ITS PHYTOCHEMICAL SCREENING

Sachin Annasaheb Nitave*, Vishin Ashish Patil

Abstract

Punica Granatum belongs to family Punicaceae. The objective of the present work was to identify the phytochemical constituents and to study of antibacterial and antifungal activity of pomegranate peel extract. Punica granatum are utilized by local people as the part of their meal and treat health diseases. Peoples supposed to consume Punica granatum seed and their peel was thrown as waste. Recently, natural products have been evaluated as sources of antimicrobial and antifungal agents with efficacies against a variety of microorganisms. Present study was designed to evaluate the antibacterial and antifungal activity of Punica granatum peel against human pathogens. The extract was prepared using ethanol. Antimicrobial activity was tested against one gram positive bacteria and three gram negative bacteria while antifungal activity was tested against two fungi. The various Concentrations (30, 60, 100mg/ml) of the ethanolic extract were tested. Evaluations were based on the zone of inhibition using Agar well diffusion assay. The inhibitory activity was found to be dose dependent. This study represents that ethanol extracts of waste material (peel) of Punica granatum may be utilize as a potential source of antimicrobial and antifungal agents. Ciprofloxacin (10μg/ml) used as standard for antibacterial activity while fluconazole (10μg/ml) used as standard for antifungal activity. The phytochemical investigation showed the presence of active chemical constituents such as alkaloids, tannins, flavanoids, steroids, cardiac glycosides and terpenoids.

Keywords: Punica Granatum, Antibacterial, Antifungal, Phytochemicals, Extraction.