Abstract

FORMULATION DEVELOPMENT AND EVALUATION OF PHOSPHOLIPID COMPLEX OF SMILAX CHINA EXTRACT FOR EFFECTIVE TREATMENT OF DIABETES

Nirmla Tiwari*, Jagdish Chandra Rathi and Rahul Sharma

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Abstract

The powdered plant material (crude drug) contains active chemical constituents which are responsible for its biological activity. Extractive value determines the approximate measure of their chemical constituents in a given amount of plant material. % Yield of Pet. ether and Hydroalcoholic extract of Smilax china were found 2.58 and 5.76% respectively. In present study total phenol content was found 0.756mg/100mg, gallic acid equivalent in hydroalcoholic extract. Different formulation of phospholipids complex of hydroalcoholic extract of Smilax china Linn were prepared different ratio of phospholipids and cholesterol and evaluated for Microscopic observation of prepared Phytosome, Drug Excipient compatibility study by FT-IR, Entrapment efficiency, Particle size and size distribution, Transmission Electron Microscopy, In-vitro dissolution rate studies and Stability studies. The entrapment efficiency of the phospholipids complex was found in the range of 45.65±0.25 to 69.98±0.14%. Particle size of all formulations found within range 245.23±0.63-355.25±0.32nm. Acarbose at a concentration of (100-500μg/ml) showed α- amylase inhibitory activity from 51.19 to 88.75% with an IC50 value 35.33μg/ml and prepared Phospholipids complex showed the IC50 value of 444.63 μg/ml. This indicates that the Phospholipids complex of Smilax china Linn can be potent α amylase inhibitor in comparison with acarbose. stability studies clearly indicates that optimized batches of phospholipids complex were stable over the chosen temperature and humidity conditions up to 3 months as were found no significant variation in physical appearance and % drug content.

Keywords: Phospholipid Complex, Smilax China, Extract, Diabete.