Abstract

A STUDY TO EVALUATE THE ANTICANCER ACTIVITY OF BARLERIA GRANDIFLORA DALZ (BG): AN IN VIVO STUDY

Dr. Jayanthi M.K*, Mr. Sunil Kumar, Dr. Sushma Naidu V, Dr. Pramod,
Manjula SN, Mruthunjaya K

Abstract

One of the best approaches in searching novel anticancer agents from plant resources is selection of plants based on ethnomedical practices and testing their efficacy and safety in light of modern science. Various parts of Barleria grandiflora dalz (BG) have been traditionally used as ethnomedicine for a number of disorders. The aim of the present study is Evaluation of Anticancer Activity of Barleria grandiflora dalz (BG) in Experimental Animals: An In-vivo Study. The Ethanol extract of leaves of Barleria grandiflora dalz was prepared by Soxhlet extraction. The in-vivo anticancer activity of BG extract was investigated in EAC induced Ascites tumor model. The toxicity of drug before starting the in vivo was checked by acute toxicity test, where the MTD was seen more than 5000mg/kg body weight in Swiss Albino rats. The In-vivo Anticancer activity was assessed by administering 250mg and 500mg/kg of BG extract orally for 9 days and Cisplatin (3.5mg/kg, i.p., Single dose). The invivo activity of BG 500mg/kg was shown more significant than the BG 250mg/kg. Hence 500mg/kg was taken for combination study with standard drug Cisplatin. The different parameters like Mean Survival Time, Percentage Increase in Life Span were restored towards normal and significant reduction in tumor weight was observed in 500mg and combination group as compared to control. The Biochemical parameters and Hematological parameters were also restored to normal.

Keywords: Barleria grandiflora, anticancer, Cisplatin, EAC induced Ascites tumor model.