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Abstract

ANALYSIS OF VAGINAL MICROBIOME COMPOSITION OF IGBO WOMEN OF REPRODUCTIVE AGE WITH AND WITHOUT SYMPTOMS OF VAGINAL DYSBIOSIS

*Onyeulor P. N., Nwaokorie F. O. and Emele F. E.

Abstract

The vagina of healthy asymptomatic reproductive aged woman is often dominated by species of Lactobacillus occupying over 90% of the bacteria composition. A shift from this (dysbiosis) may result to myriad of health complications. Vaginal dysbiosis is often diagnosed using clinical symptoms. Are all women without clinical manifestation of vaginal dysbiosis apparently healthy? Can the diagnosis of vaginal dysbiosis be based on clinical manifestations? This study is aimed at ascertaining the vaginal bacteria composition of women with and without clinical manifestations of vaginal dysbiosis using new generation sequencing (NGS) in order to ascertain the role of clinical symptoms in the diagnosis of vaginal dysbiosis. A total of 15 respondents were recruited for the study. Speculum assisted High vaginal swab (HVS) samples from posterior fornix of each respondent were collected, the DNA of the microorganisms from the HVS were extracted, purified, quantified and V3-V4 hyper variable region of 16S rRNA was amplified using paired end bar-coded universal primers which was thereafter sequenced using illumina miseq platform. Sequence reads were quality filtered and Chimeras were removed. Sequence reads with 97% similarity were assigned to the same operational taxonomic unit (OTU) using quantity insight into microbial ecology (QIIME). National centre for biotechnology information (NCBI) database was used to identify the OTUS. Total number of phylum obtained from women with symptoms were five with Firmicutes having the highest prevalence with relative abundance of 83.8%, Actinobacteria had 14.1%, Proteobacteria, 5.2%, Bacteriodetes 3.3% and Cyanobacteria recording 1.9% while a total of six phyla were obtained from women without symptoms, with Firmicutes recording 61.2%, Fusobacteria 41.1%, Proteobacteria 27.0%, Bacteriodetes 15.5%, Actinobacteria 12.2% and Tenericus recording 2.0%. Hundred percent (100%) of symptomatic participants had high lactobacillus species abundance and low level of facultative anaerobes. Hundred percent (100%) of participants which were from symptomatic participants with clinical symptoms of infection had high lactobacillus species abundance while 36.4% (4/11) of asymptomatic participants had high level of lactobacillus species with 18.1% (2/11) having low lactobacillus species while 45.5% (5/11) had no lactobacillus species and high level of facultative anaerobes. Conclusively, clinical symptoms of vaginal dysbiosis do not depict the state of the diversity and abundance of vaginal microbiota of reproductive aged Igbo women hence cannot be used as an indicator of vaginal dysbiosis.

Keywords: Dysbiosis, Microbiome, Microbiota, Symptoms, Vaginosis.


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