DEVELOPMENT AND VALIDATION OF UFLC METHOD FOR QUANTIFICATION OF FLAVONOIDS IN TECTONA GRANDIS LEAVES
Durgesh Nandkishor Boob*, Sonali Niraj Choudhary and Sanman Sitaram Kolhe
Abstract
A simple Ultra fast liquid chromatography (UFLC) method for the
separation and quantitative determination of the flavonoids from
Leaves of Tectona grandis, was developed. UFLC is a new technique
in LC offering several potential advantages, especially the reduction of
time. A special UFLC analysis performed on shimadzu LC- 30 AD
liquid chromatograph equipped with column Aquity C18 (100 X
2.1mm) with a particle size of 1.7μm. The method was validated to
demonstrate its specificity, Accuracy, Precision, LOD, LOQ, Linearity,
Range, robustness. For rutin and quercetin the method was validated
according to ICH guidelines. Method was highly precise and accurate
because it shows low relative standard deviation as well as good % recovery values.
Keywords: Tectona grandis, flavonoids, Rutin, Quercetin, UFLC, Validation.
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