Abstract

DEVELOPMENT AND VALIDATION OF UFLC METHOD FOR QUANTIFICATION OF FLAVONOIDS IN TECTONA GRANDIS LEAVES

Durgesh Nandkishor Boob*, Sonali Niraj Choudhary and Sanman Sitaram Kolhe

Abstract

A simple Ultra fast liquid chromatography (UFLC) method for the separation and quantitative determination of the flavonoids from Leaves of Tectona grandis, was developed. UFLC is a new technique in LC offering several potential advantages, especially the reduction of time. A special UFLC analysis performed on shimadzu LC- 30 AD liquid chromatograph equipped with column Aquity C18 (100 X 2.1mm) with a particle size of 1.7μm. The method was validated to demonstrate its specificity, Accuracy, Precision, LOD, LOQ, Linearity, Range, robustness. For rutin and quercetin the method was validated according to ICH guidelines. Method was highly precise and accurate because it shows low relative standard deviation as well as good % recovery values.

Keywords: Tectona grandis, flavonoids, Rutin, Quercetin, UFLC, Validation.