Abstract

ISOLATION AND IDENTIFICATION OF PROTEASE FROM BACILLUS SPECIES USING SDS-PAGE

Huda Jameel Makki, Ganesh Dama, Prasad M.P, Balasubramanian Sathyamurthy*

Abstract

This investigation was carried out to isolate and identification of protease producing bacteria from different sources like soil, water and fruit samples. The colonies producing protease were identified as Bacillus strains by standard microbiological techniques. The Bacillus strains were grown on nutrient broth for the isolation of protein. The isolated proteins were precipitated using ammonium sulphate. The proteins got precipitated at 50% and 60% saturation of ammonium sulphate. Eluted proteins were then identified using SDS-PAGE with standard protein marker. SDS-PAGE results shown, with 50% ammonium sulphate precipitation showed high amount of proteins with multiple bandings with different molecular weights and with 60% ammonium sulphate precipitation showed low amount of proteins as They had faint bands with multiple bandings pattern of different molecular weights.

Keywords: Bacillus, Protease, SDS-PAGE, Proteins, Microbiological techniques.