Abstract

DEVELOPMENT, CHARACTERIZATION AND OPTIMIZATION OF GENTAMYCIN SULFATE LOADED BONE GRAFT

Prothibha Das*, Arunima Aravind, Ajith Babu T. K. and Rahina A. S.

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Abstract

Bone defects occur in a wide variety of clinical situations and their reconstruction to provide mechanical integrity to the skeleton is a necessary step in the patient‟s rehabilitation. The different approaches for bone reconstruction by autografts (transplanted directly from one area of the individual skeleton) and allografts (comes from a donor) has some disadvantages such as the need for a second surgery and the limited quantity and shape of available bone and chances of disease transmission. Here gentamicin sulfate loaded bone grafting material was prepared using dissolution method. The formulation was optimized using Box – Behnken design keeping chitosan, citric acid, and HPMC levels as independent variables. The responses obtained from the design matrix i.e. percentage porosity and mechanical strength were statistically evaluated and an optimum formula was suggested by the design expert software. The setting time of the prepared material was also determined and this parameter was inversely proportional to the number of polymers and the amount of citric acid. The invitro antibacterial study was done using Staphylococus aureus by well method and formulation has good zone of inhibition.

Keywords: Gentamycin, Bone grafts, Box-Behnken, Bone substitute.