Abstract

ANTIMICROBIAL ACTIVITY OF CERTAIN ANTIBIOTICS ON THE MICROBIAL ISOLATES OF KERATITIS PATIENT

V. Nithya*Anusha Baskar, M.H. Muhammad Ilyas

Abstract

Microbial keratitis is a major cause of monocular blindness in developing countries. Keratitis caused by bacteria, viruses, fungi and parasites. To investigate the identification of bacteria and fungi from keratitis patients using certain antibiotics. The samples were examined in the Eye Clinic of Annal Gandhi Memorial Government, Hospital, Tiruchirappalli, India. All patients were examined with a slit lamp biomicroscope. The bacterial and fungal colonies were isolated in eye swabs of keratitis patients and were identified by using biochemical tests. In this study, the antimicrobial activity of several antibiotics was evaluated using micro dilution and antimicrobial susceptibility tested against clinically isolated microorganisms from keratitis patients. The antimicrobial activity for Staphylococcus aureus, Streptococcus viridians, Pseudomonas aeuoroginosa, Staphylococcus epidermis, Candida albicans and Aspergillus tubingensis against various antibiotics such as Gentamycin, Ampicilin, Tetracycline, Ciprofloxacin, Nystatin, Flucytosine, Amphotericin and Miconazole were studied. Most of the bacteria were sensitive to Gentamycin, Ampicillin, and Tetracycline whereas the fungi like Candida albicans and Aspergillus tubingensis were sensitive to Nystatin and Flucytosine. In the present study, all bacteria and fungi were found to be sensitive to Gentamycin, Ampicilin, Nystatin and Flucytosine.

Keywords: Microbial keratitis, Bacteria, Fungi, Antibiotics.