Abstract

ANTIBACTERIAL ACTIVITY OF JUNIPERUS COMMUNIS L. AND VITEX NEGUNDO AGAINST XANTHOMONAS AXONOPODIS PV PUNICAE IN VITRO

Digvijay*, Satyavrat Bhardwaj, Pankaj Kumar

Abstract

Leaf solvent extracts of Juniperus communis L. and Vitex negundo were prepared in different solvents (methanolic, ethanolic, petroleum ether and water) and were evaluated for antibacterial activity against Xanthomonas axonopodis pv punicae in vitro. Different working concentrations for each extract were prepared separately viz. 20, 40, 60, 80, 100, 200, 300 ppm and evaluated for their antibacterial activity at 24, 48 and 72 hours respectively. Among these plants extracts concentrations methanolic and ethanolic extracts at 300ppm were more effective than that of other extracts concentrations for both plants. The maximum zone of inhibition was found to be exhibited by ethanolic extract of Juniperus communis L. (21 mm) and methanolic extract of Vitex negundo (26 mm) at 300 ppm concentration after 48 hour and 72 hours respectively. All the plant extracts show inhibitory effect on linear growth of Xanthomonas axonopodis pv. punicae.

Keywords: Medicinal plants, Antibacterial activity, zone of inhibition, Xanthomonas axonopodis pv. punicae.