Abstract

A VALIDATED RP-HPLC METHOD FOR THE SIMULTANEOUS DETERMINATION OF LEVODOPA, CARBIDOPA AND ENTACAPONE IN TABLET DOSAGE FORMS

Gandla. Kumara Swamy*, JM Rajendra Kumar , J.V.L.N.Seshagiri Rao

Abstract

A Stability indicating RP-HPLC method has been developed for thesimultaneous estimation of Levodopa, Carbidopa and Entacapone intablet dosage forms using an RP- Hypersil BDS column (150 x 4.6mm; 5). A mobile phase consisting of Potassium Dihydrogenorthophosphate and Acetonitrile in the ratio of 95:5.0 was pumpedthrough the column at a flow rate of 1.0 mL/min. The separations ofdrugs were monitored at 280 nm. Linearities of quantification wereobserved in the concentration range of 75–225 μg/ml for Levodopa,18.75-56.25 μg/ml for Carbidopa and 100 -300 μg/ml for Entacapone.The method was duly validated. A%RSD of less than 2% indicates thehigh degree of accuracy and precision of the method. Due to its highprecision and accuracy, the proposed Stability indicating RP-HPLCmethod can be applied for determining Levodopa, Carbidopa andEntacapone simultaneously in bulk and Combined Tablet dosageforms.

Keywords: Levodopa, Carbidopa and Entacapone; RP-HPLC Method; Forced Degradation studies; Tablet dosage forms.