Abstract

PHYTOCHEMICAL ANALYSIS AND ANTIPROLIPHERATIVE ACTIVITY OF MURRAYA PANICULATA LEAVES AGAINST MDAMB- 231 CELL LINE

Shreya Biswas and Shila Elizabeth Besra*

Abstract

We studied the phytochemical constituents and antiproliferative activity of methanolic extract of Murraya paniculata leaves (MEMPL) on breast carcinoma cell line. The MTT assay showed, MEMPL significantly inhibited cell growth in a time and concentration dependent manner in the MDA-MB-231 cell line. In morphological study fluorescence microscopy images of MEMPL treated cells showed several signs of apoptosis than control cells. The further morphological study by confocal microscopy images showed condensed chromatin and fragmented nuclei as apoptotic bodies in treated cells than untreated control cells. The agarose gel electrophoresis study showed degraded DNA bands in carcinoma cells than control. These findings suggest that Murraya paniculata leaves can be a potential anti-carcinoma therapeutic agent in future.

Keywords: Murraya paniculata, Leaf, Cancer, Breast, Cytotoxic.