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Abstract

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION BY RP-HPLC: A REVIEW

Vaibhav S. Chikhale*, Dr. N. G. Sutar

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Abstract

A straightforward, exact, precise, explicit and RP-HPLC technique was created for assurance of medication in drug definition. The introduced study is straightforward, since tests are straightforwardly utilized with next to no primer substance sensation or purification steps. For the most part a RP-HPLC test use Evenness C18 or comparable with versatile stage structure of ph 7 buffer: acetonitrile was utilized, and stream rate was 0.8 ml min-1 with UV identification at 285 nm. The proposed strategy was approved for different ICH boundaries like linearity, breaking point of discovery, accuracy, precision, ruggedness, robustness, also framework reasonableness.

Keywords: RP-HPLC, Chromatography, validation, mobile phase, reverse phase.


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