Abstract

ANTIOXIDANT ACTIVITY AS FREE RADICAL SCAVENGING EFFICACY OF AYURVEDIC DENTIFRICE

Jyoti Singh*, Amit Sirdesai and Dr. Prasun Bandyopadhyay

Abstract

The oral cavity is under constantly bombardment from foods and drinks, substances such as alcohol, medicines, tobacco products, nicotine, dental materials and much more. Bacteria and other disease-causing agents along with systemic conditions present further insult. Because the oral tissues are delicate, they are especially vulnerable to cell damage caused by free radicals and oxidative stress. Maintaining a good balance of oxidants and antioxidants is very essential for maintaining oral health. Antioxidants help to offset this imbalance. Antioxidants can help adjunct treating the progress of oral problems such as periodontitis or gingivitis, and other gum health issues. The present study was conducted to evaluate the free radical scavenging activity of Dabur Red Paste & Non-Herbal fluoridated toothpaste using DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay using in vitro method. L-Ascorbic acid (vitamin C) was used as a positive control, which is well reported for its antioxidant property. Dabur Red Paste & Non-Herbal fluoridated toothpaste were tested for antioxidant potential at various concentrations ranging from 0.1 % – 10 %. Results demonstrated that Dabur Red Paste in the concentration 0.1- 10%, displayed free radical scavenging activity by maximum of 78% & 76% at 30 min & 2 min incubation respectively. However, no free radical scavenging activity was observed in Non-Herbal Fluoridated Toothpaste. The data obtained from the present study revealed that the test item Dabur Red Paste possesses antioxidant activity with IC50 (Inhibition Concentration at 50 %) value of 1.0 % at 2 min incubation which is minimum recommended time duration for brushing.

Keywords: Antioxidants, Free radicals, Periodontal disease, Oral cavity, DPPH assay, Dabur Red Paste.