Abstract

THE GROWTH SUPPRESSING EFFECTS OF LEAF EXTRACT OF BISCHOFIA JAVANICA BLUME ON GLIOMA CELLS

Shila Elizabeth Besra*, Sutharson Lingadurai and J. R. Vedasiromoni

Abstract

Natural products have long been known as one of the most excellent sources of lead drug candidate in drug discovery. Nowadays, a large number of Indian medicinal plants have been utilized for the treatment of a variety of diseases. Leaves of Bischofia javanica have been traditionally used for many ailments including cancer. Anti-leukemic activity of the leaf extract was already reported on human leukemic cell lines The present study investigated the cell cycle arrest and apoptosis activity of methanolic extract of Biscofia javanica Blume on glioma cell lines. The extract significantly inhibited cell viability in a time and concentration dependent manner in both the glioma cells. Morphological study showed several signs of apoptosis like chromatin condensation, nuclear fragmentation and formation of apoptotic bodies and degraded DNA bands in carcinoma cells than control. Cell cycle phase arrest was observed in the G0/G1 phase and MMP shift assay exhibited significant change in the methanolic extract of Biscofia javanica (MEBJ) treated cells. These findings suggest that metabolic activity of both the glioma cells were suppressed by extract and possesses induction of apoptosis and cell cycle arrest.

Keywords: Bischofia javanica, Glioma, Cell Cycle, Apoptosis.