Abstract

HPTLC FINGERPRINTING STUDY OF HYDROALCOHOLIC EXTRACT FOR ALKALOIDS FROM Ailanthus excelsa (Roxb.) LEAVES

Mudit Kumar*, Amita Tilak and Sudhir Singh Gangwar

Abstract

Objective: The present study was conducted to identify the alkaloids from Hydroalcohol extract of medicinally and economically useful leaves of Ailanthus excelsam (Roxb.) using High Performance Thin Layer Chromatography (HPTLC) technique. Methods: Preliminary phytochemical screening was done and HPTLC studies were carried out. CAMAG HPTLC system equipped with Linomat V applicator (Switzerland). Densitometric scanning was performed with Camag TLC scanner IV in the reflectance absorbance mode at 540 nm and operated by Win CATS software (1.4.6 Camag) with the help of tungstant lamp. Results: HPTLC finger printing of alkaloids of Hydroalcohol extract of leaves shows absence of alkaloids as no orange bands were seen after derivatisation with Dragendorff reagent. Conclusions: With the results of HPTLC analysis we have concluded the absence of alkaloids in the extract.

Keywords: Ailanthus excelsa (Roxb.) leaves, alkaloids, HPTLC Fingerprinting.