Abstract

ANALYZING THE ANTIBACTERIAL ACTIVITY OF CHITOSAN HYDROGEL COATED ALOE VERA PULP (CS-AV) AGAINST AEROBIC AND ANAEROBIC PATHOGENIC BACTERIA – IN VITRO

Fatima Redah Alassaif, Eman Redah Alassaif, Amit Kumar Kaushik and Jeevitha Dhanapal*

Abstract

The current article aimed to evaluate in vitro antibacterial activity of chitosan hydrogel coated Aloe vera pulp (CS-AV) using TPP as a cross linking agent. The hydrogel was prepared in the mixture of chitosan (CS) with the concentration of (2% w/v) in acetic acid (0.5%, v/v), TPP (0.01% w/w) and Aloe vera (AV) pulp (0.5% v/v). The morphology of the CS-AV hydrogels showed that the hydrogels have porous structures and interconnected pores. The stability of the hydrogels showed that the hydrogels could swell up to 180 % of their initial weight during 6 h and loss 28.2 ± 4.7% of the initial weight during 14 days. The antibacterial studies depicted that the prepared CS-AV hydrogel inhibited the growth of S. aureus, P. aeruginosa, K. pneumoniae, E. coli, and S. pyogenes and their minimum inhibition concentration was also estimated. Further in vivo studies need to be estimated.

Keywords: Antibacterial; Aloe vera; Chitosan; TPP; Hydrogel.