Abstract

HISTOMORPHOMETRIC COMPARISON ON DOXO-TOXICITY WITH SAFETY EVALUATION OF ANTIOXIDANTS WITH VITAMIN C IN ALBINO RAT TESTES

Farooq Khan*, Nighat Ara, Rifat Shamim, Rizwan Ul Haq, Shamila Hafizi and Ahmad Nabi

Abstract

Objective: To observe the histological effect of conjoint antioxidant in comparison to vitamin C on the doxo-toxicity induced on rat testes. Methodology: The following experimental randomized control analysis research was conducted on male albino rats. This study was conducted in the PCSIR laboratory complex and in Peshawar medical college during February- July 2019. The Rats were arbitrary divided into two major groups. The normal and the experimental groups. Experimental group was further subdivided into toxic (B-I), conjoint group (B-II) and Vitamin C (B-III) groups. The normal group animals were given i.p. injection of placebo on weekly basis, while in the experimental group B-I group was introduced to doxorubicin at a dose of 02 mg/kg i.p. body weight weekly for four weeks. While the conjoint group (B-II) were given i.p. of same dose with combine therapy of Vit. C and E orally on daily basis. While the vitamin C (B-III) groups were given orally on daily basis. After oblations of the animal were used according to the protocol, the testes were taken out and sectioned. The prepared slides were used for the basic stains i.e: H and E, Periodic Acid Schiff stain and Massan Trichrome stains. Data of the experimental groups was analyised by the SPSS version 21, and the P value was considerably significant statistically. Results: The current research was conducted to observe the doxo-toxicity in Albino rats, and to find out the possible protective therapies in combination in contrast to vitamin C. B-I group treated with Doxorubicin only manifest notable decreased body weight, testicular weight, reduced height of seminiferous epithelium and count of germ cells as compared to B-II and B-III. Also there were positive and good results in conjoint therapy comparable to single antioxidants. Conclusion: It is concluded that simultaneous use of antioxidants comparable to single, can shows better results to prevent damage to testes.

Keywords: Doxorubicin, Testicular toxicity, Histomorphometery, vitamin C, Vitamin E.