Abstract

IN VITRO EVALUATION OF METHOTREXATE LOADED WITH SOLID LIPID NANOPARTICLES FOR BREAST CANCER

Vaibhav Vaishnav*, Dr. Anand Mahalwar, Ashwanee Kumar Sahu, Yugal Kishor Rajput, Rajendra Kumar Sahu and Omprakash Sahu

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Abstract

Breast cancer is cancer that develops from breast tissue lump include in breast cancer, change in breast shap and skin is dimpling, it is the most common type breast cancer is ductal carcinoma. The present study is designed to methotrexate loaded with solid lipid nanoparticles (slns) to target Breast cancer. The developed nano-carriers where characterized with respect to particle size, PDI, zeta potential, drug loading and entrapment, in vitro releas etc. The characterized formulation were used to comparatively assess cellular uptake, cellviability, apoptosis, lysomal membrane permeability, bioavailability, biodidtsribution, changes in tumer volume in anumal survival. The results ex-vivo showed greater cellular uptake and better cytotoxicity at lower IC50 of methotrexate in breast cancer cells. Further, we observed increased programmed cell death (apoptosis) with altered lysosomal membrane permeability and better rate of degradation of lysosomal membrane in-vitro. On the other hand, in-vivo evaluation showed maximum bioavailability and tumor targeting efficiency with minimum secondary drug distribution in various organs with formulated and anchored nano-carrier when compared with free drug. Moreover, sizeable reduction in tumor burden was estimated with fucose decorated SLNs as compared to that seen with free MTX and SLNs-MTX. Fucose decorated SLNs showed promising results to develop therapeutic interventions for breast cancer, and paved a way to explore this promising and novel nano-carrier which enables to address breast cancer.

Keywords: Brest cancer, Methotrexate, Solid lipid nano particle. Targeted delivery.