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Abstract

BIOLOGICAL EVALUATION OF CRUDE ALKALOID EXTRACTS OF NYCTANTHES ARBORTRISTIS AND ARISTOLOCHIA INDICA

Usha Rani Pradhan, Sumit Sarangi and Sunita Bhatnagar*

Abstract

Nyctanthus arbortristis and Aristolochia indica are popular plants in the Ayurvedic system of medicines. In the present study two different protocols of alkaloid isolation from the leaves of the plant were taken up and their biological activity was evaluated using brine shrimp assay and Thin liquid chromatographic analysis. Crude alkaloids isolated from the second protocol showed 100% activity against brine shrimp larvae in case of both the plants. All the extracts showed chromatographic separation in different solvents, best separation was obtained in benzene solvent.

Keywords: Nyctanthus arbortristis, Alkaloids, brine shrimp assay, TLC.


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