Abstract

APPLICATIONS OF CRISPR/CAS9 IN IMMUNOTHERAPY

Harshita Goswami, Kanthesh B. M.* and Gopenath T. S.

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Abstract

Cancer is among the deadliest diseases worldwide, though years of studies and research has made the understanding of the tumor cells really conventional helping to inculcate various treatment options, immunotherapy is one of the advanced options for reversing the tumor activity, and implementing CRISPR/cas9 in the gene editing with best possible results is taking onco-immunology to the next extent. Various genes are associated with the negative regulation of the CAR cells which are essential in T-cell therapy. These genes like, B2M, PD-1, TRAC, LAG-3 can be knocked down very precisely by easy to use and immensely prompting gene edited technique of CRISPR/cas9. Various studies have been conducted incorporating CRISPR/cas9 in enhancing the scope of immunotherapy. This review is subjected to study the genes and their downregulation by CRISPR/cas9 and challenges this technique is still holding along with the future implementations to well orient oncology along with immunology helping in the eradication of cancer.

Keywords: CRISPR/cas9, immunotherapy, PD-1, LAG-3, lung cancer.