Abstract

CYTOTOXIC EFFECT OF TRICHOSANTHES DIOICA WALL. LEAVES ON COLORECTAL CARCINOMA CELLS AND CELL CYCLE ARREST

Sabyasachi Choudhuri and Shila Elizabeth Besra*

Abstract

We have evaluated the anti-glioma effect of Trichosanthes dioica leaves in our previous paper. Colorectal cancer (CRC) is the third most frequent malignancy and the fourth leading cause of death from cancer. Therefore, we studied the effect of methanolic extract of Trichosanthes dioica leaves and its fraction (METDL, EFTDL & BFTDL) on colorectal carcinoma cells. The extract and its fraction significantly inhibited cell viability in a concentration dependent manner in HCT 116 cell line. Confocal microscopic study showed nuclear fragmentation in EFTDL treated carcinoma cells than control. Cell cycle phase arrest was observed in the G0/G1 phase in the EFTDL treated cells. These findings suggest that Trichosanthes dioica leaf can be a potential anti-carcinoma therapeutic agent for colorectal carcinoma.

Keywords: Trichosanthes dioica, Leaves, Colorectal Cancer, Cell Cycle.