Abstract

HEN'S EGG TEST – CHORIOALLANTOIC MEMBRANE (HET-CAM) TEST METHOD FOR EXAMINATION OF DERMAL OR CUTANEOUS IRRITATION OF ME.CP AS A POLYMER

Prof. Saurabh Dilip Bhandare* and Dr. G. S. Deokar

Abstract

Hen's egg–chorioallantoic membranes were used to screen for, information access and, evaluate anti-irritant properties of aqueous extracts of plants (HET–CAM tests), plant derived polymer gels, gums and, other acidic and alcoholic extracts in connection with searches for plant-derived substances that have topical anti-irritant action. Applicable in the medicinal chemistry in finding the potential drug effects in testing its teratogenic effect and, irritation in gastric or dermal membranes. This evaluation mainly focus on CAM-assay screening of plant extracts which could provide a useful information for identifying promising anti-irritant nature of plant aqueous, alcoholic, ethanolic, acetone extracts, exudate, gum, resins or polysaccharides, for the follow-up on clinical testing. The method would require materials with strong anti-irritant properties, and, would also have to avoid registering false negative data. The involved tests that were conducted, provided positive indications. Examination and evaluation of the delays in the onset of three manifestations of significant membrane irritation–that includes: 1. vascular haemorrhaging, 2. membrane lysis and, 3. membrane coagulation; observed with the test substances (t.s.), in relative to the positive controls. Aqueous 10% laboratory prepared irritant, a commonly generative of skin irritation in the animal or human was used as irritant in the direct test as a standard; employed as the test irritant in this study which was evaluated. The ratio [irritation onset times after test substance pre-treatment]:[onset times without test substance pre-treatment] was used to measure the anti-irritant and if any irritation inducing capacity of the test substances before starting any preclinical study and, evaluation of any significant measures.

Keywords: HET-CAM, chicken cell embryo testing, irritation testing, skin irritation evaluation, artificial embryogenesis.