Abstract

GC-MS ANALYSIS OF ETHANOLIC INFLORESCENCE EXTRACT OF Musa Paradisiaca L. (AB)

Athira Anilan*, Silpa I.S., Sona C.A., Gopika K.S., Mohamed Fuaad Bin Haneefa, Arppitha Jayakumar

Abstract

GC-MS is an analytical method used for the isolation and identification of volatile constituents.The aim of the study is to identify the bioactive phytochemicals present in the ethanolic extract of spadix inflorescences of Musa paradisiaca L (AB) by Gas chromatography and Mass spectroscopy (GC-MS). The plant is commonly known as kunnan vazha (Kannan varieties) in Malayalam.GC MS analysis of ethanolic extract was done by Gas Chromatography–Mass Spectrometry using SOP, while the extracted compounds was matched with libraries of NIST 11 & WILEY 8. The GC-MS analysis showed the presence of eleven (11) compounds in Musa paradisiaca L. (AB). The identified constituents were found to be biologically active. Methods: The dried and coarsely powdered plant materials were extracted using solvent ethanol for 6 hours by soxhlet extraction method. The presence of various phytochemical constituent was analyzed by GC-MS. Results: Totally 11 chemical constituents were identified. The detected eleven compounds from ethanolic extract were Z- 12-Pentacosene, Pentacosane, n-Tetracosanol-1, Octacosane, 1-Heptacosanol, 17- Pentatricontene, Oleic acid, Propyl acid,22-Desoxycarpesterol, gamma-sitosterol, Cycloartenyl acetate, Cycloeucalenyl acetate and were identified as therapeutically active. Conclusion: The obtained phytochemical constituents were identified as potentially active and can be used for the treatment of various diseases. Constituents supporting anti-oxidant activity were also identified.

Keywords: Musa paradisiaca L. (AB), GC-MS, Soxhlet extraction.