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Abstract

ISOLATION, PURIFICATION OF VENOM TOXINS/PEPTIDES FROM PAPER WASP ROPALIDIA MARGINATA AND ITS EFFECTS ON VARIOUS HEMATOLOGICAL PARAMETERS IN ALBINO MICE

Simran Sharma* and Ravi Kant Upadhyay

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Abstract

In present research study paper wasp Ropalidia marginata Venom toxins were purified on Sepharose CL 6B 200 gel column at a constant flow rate of 5 mL/minute in 121 regular fractions. Elution pattern of venom shows presence of low molecular weight toxic peptides ranging from 12-60 kDa. Eluted fractions were pooled lyophilized and its LD50 was determined in albino mice. The LD50 of the venom protein was Ropalidia marginata found 20.6± 0.094 mg/kilogram body weight of albino mice. Ropalidia marginata toxins/proteins caused significant (p

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