Abstract

IN-VITRO IMMUNOMODULATORY ACTIVITY OF SIDDHA FORMULATION ATHIMATHURA CHOORANAM (AMC) IN RAW MACROPHAGE CELL LINE

Arthi G.*, Lakshmana Kumar V., Rathinam J. and Sushil Kumar P. N.

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Abstract

Athimathuram is one of the most important herb Glycyrrhiza glabra Linn. The word Glycyrrhiza is derived from Greek term glykos (meaning sweet) and rrhiza (meaning root). Glycyrrhiza glabra Linn belongs to Leguminosae / Fabaceae family. Historically mulethi/liquorice has been used from more than 4000 years. Athimathura Chooranam (AMC) Glycyrrhiza glabra Linn Root powder is the Siddha formulation which is used for many centuries to treat various ailments such as Venkuttam(Vitiligo), Kalanjagapadai (Psoriasis) Karappan(Eczema) from time immemorial. Here we report the immunomodulatory activity of AMC in-vitro Raw Macrophage Cell Line. For this study, Lipopolysaccharide (LPS) stimulated RAW cells were exposed with different concentration of the AMC incubated Immunomodulatory response was performed by estimating nitrite levels in the cell lysate. The level of nitrite level was estimated by the method of Lee et al. (Lepoivre et. al. 1990). It was observed that there was dose dependent decrease in the nitrite level in RAW 264.7 medium incubated with test drug at the concentration ranges from 50 to 200 μg/ml. LPS (1μg/mL) treated well was served as a control with maximum nitrite level of about 1261 ± 52.77μg and the maximum percentage decrease of nitrite level of about 390.7 ± 78μg were observed at 200 μg/ml. In this study based on in-vitro experiments reveals that AMC having Immunomodulatory activity. So it can be treated for auto immune disease like Vitiligo (Venkuttam) Kalanjagapadai (Psoriasis) Karappan (Eczema) is used worthwhile.

Keywords: Athimathuram, Glycyrrhiza glabra Linn, Immunomodulatory, Cell line study, Siddha.