WJPR Citation New

  All Since 2019
 Citation  7475  4312
 h-index  28  21
 i10-index  197  83

Login

Best Paper Awards

World Journal of Pharmaceutical Research (WJPR) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.
Best Paper Award :
Dr. Dhrubo Jyoti Sen
Download Article: Click Here

Search

Track Your Article

Abstract

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF DAPAGLIFLOZIN AND TENELIGLIPTIN HYDROBROMIDE HYDRATE FROM SYNTHETIC MIXTURE BY THREE DIFFERENT UV SPECTROPHOTOMETRIC METHODS

Anokhi Patel*, Preeti Jadeja and Dr. Rajashree Mashru

.

Abstract

Three new UV spectrophotometric methods namely simultaneous equation, Q-absorbance ratio and first derivative (zero crossing) spectroscopic methods were developed and validated for simultaneous estimation of Dapagliflozin and Teneligliptin hydrobromide hydrate which were simple, sensitive, precise and accurate. In simultaneous equation method, absorbance was measured at 223 and 243 nm for both the drugs. Dapagliflozin and Teneligliptin hydrobromide hydrate hydrochloride showed an iso-absorptive point at 230 nm in Qabsorbance ratio method. The second wavelength used was 223 nm which is λ max of Dapagliflozin in distilled water. First derivative (zero crossing) method was based on the transformation of UV spectra in to first derivative spectra followed by measurement of first derivative signal at 223 and 243 nm for Dapagliflozin and teneligliptin hydrobromide hydrate, respectively using 2 nm as wavelength interval (Δλ) and 1 as scaling factor. Developed methods were validated according to ICH guidelines including parameters viz., specificity, linearity and range, precision, accuracy, limit of detection and quantification. All the three methods showed linear response in the concentration range of 5-75 μg/ml for both the drugs. Results of method validation parameters follows ICH guideline acceptable limits. Based on the assay results obtained, methods were compared using one-way ANOVA. Methods were found to be simple, fast, highly sensitive, cost effective and hence can be useful for simultaneous estimation of Dapagliflozin and teneligliptin hydrobromide hydrate which showed good applicability of the developed method.

Keywords: Dapagliflozin (DAPA) Teneligliptin (TEN) simultaneous equation, Q-absorbance ratio, first derivative (zero crossing) spectroscopic methods.


[Full Text Article]  [Download Certificate]

Call for Paper

World Journal of Pharmaceutical Research (WJPR)
Read More

Email & SMS Alert

World Journal of Pharmaceutical Research (WJPR)
Read More

Article Statistics

World Journal of Pharmaceutical Research (WJPR)
Read More

Online Submission

World Journal of Pharmaceutical Research (WJPR)
Read More