Abstract

FORMULATION AND EVALUATION OF IN SITU GEL OF LEVOFLOXACIN AND DEXAMETHASONE

Prashant Pachori*, Aditya Tiwari and Nitendra Sahu

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Abstract

In-situ gel of Levofloxacin and dexamethasone was formulated and evaluated. During the preformulation, the solubility, Melting point, Wavelength of maximum absorption (λmax) and Calibration curves were obtained of Levofloxacin and Dexamethasone. Gellan based in situ gel systems were prepared by dissolving gellan alone and its combination with sodium alginate and/or HPMC in hot phosphate buffer mannitol (5% m/V) were added as isotonicity agent. Different evaluations were carried out as visual appearance, transparency, clarity, pH, drug content, viscosity, in-vitro gelation studies, gel strengths, bioadhesion, and sterility, all evaluated parameter are under limit as required for insitu gel formation. Among the all prepared L5 has drug contain above 99% of both drugs gelation immediate and remains for an extended period, Gel Strength (peak Load) of 72g and best bio-adhesion of 35g. %Cumulative drugs release from formulation L5 in STF solutions was studied where 69.72% Levofloxacin and 72.34% Dexamethasone was released. The ophthalmic formulations must be isotonic to avoid damage to delicate tissues of eye. It is concluded that the optimized in-situ gel formulation is a better in results and evaluation parameters.

Keywords: In-situ gel, Levofloxacin, Dexamethasone, Isotonicity, Bioadhesion.