Abstract

MICROWAVE ASSISTED SYNTHESIS AND PHARMACOLOGICAL EVALUATION OF 1-(2,4-DINITRO PHENYL)-3,5-DIPHENYL 4,5- DIHYDRO PYRAZOLE

Susmitha P. V.*, Alwin Siby, Sherin Ismail, Sajna, Sachena Stephen and Wafa P.

Abstract

Chalcone compounds are unsaturated ketone involving the reactive ketoethylenic group (CO-CH=CH-). To synthesis a novel Chalcone derivative by using microwave assisted synthesis, study antibacterial activity and evaluate the zone of inhibition of each concentration of the synthesized Chalcone derivative, to evaluate in-vitro antioxidant activity by DPPH assay method. We analysed IR and mass spectral data to confirm the structure of the synthesized compound. Synthesisis was done by means of a microwave irradiation technique and synthesized 1-(2,4-Dinitrophenyl)-3,5-diphenyl 4,5-dihydro pyrazole using 2,4-dinitrophenyl hydrazine and trans chalcone along with ethanol and few drops of conc. HCl. Antibacterial studies were carried out by using agar well plate methods. Antibacterial studies showed significant zone of inhibition against Escherichia coli and Staphylococcus aureus. In vitro antioxidant study such as DPPH assay was performed and was compared with standard ascorbic acid. The significant antimicrobial activity was recognized for the synthesized Chalcone derivative including the concentration of 50,100 and 200μg/ml. When compared to ascorbic acid (standard), the chalcone derivative is effective in antioxidant activity. The functional groups present in the chalcone derivative were determined by means of IR spectroscopy. The mass spectroscopical study was carried out to determine the molecular weight which was found out as 316 mol.

Keywords: Microwave Assisted synthesis, Chalcone Derivative, Antioxidant, Antimicrobial activity, Ascorbic acid, IR, MASS.