Abstract

ANTITUBERCULAR AND ANTICANCER ACTIVITIES OF COMPOUNDS OBTAINED FROM METHANOL EXTRACT OF TOONA CILIATA LEAVES

Dr. Vinodhini Venkatesan*, Rozario Cyril and Dr. Deepa

Abstract

The inappropriate use of drugs in tuberculosis has led to the appearance of multidrug resistance and extremely drug-resistant tuberculosis. The cytotoxic drugs used in the treatment of cancer are producing many adverse drug effects. There is an increased need to find out new therapeutic compounds to overcome these problems. Toona ciliata tree belongs to the Meliaceae family and it has many traditional uses. Four compounds were isolated from the methanolic extract of Toona ciliata leaves. All the compounds were tested for antitubercular activity against sensitive, resistant, and standard strain of Mycobacterium tuberculosis using luciferase reporter phage assay. Among the four compounds, three compounds were active against the resistant strain of Mycobacterium tuberculosis. One compound is active against the standard and sensitive strains of Mycobacterium tuberculosis. The anticancer activity was tested using MTT (3-(4, 5- dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay and Annexin V/ Propidium Iodide staining using Fluorescence-activated single cell sorting (FACS) analysis. All compounds have shown good activity in the MTT assay and compounds M4 and M3 have shown more apoptotic activity.

Keywords: Toona ciliata, Meliaceae, Luciferase reporter phage assay, Mycobacterium tuberculosis.