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Abstract

ASSESSMENT OF IN-VITRO FREE RADICAL SCAVENGING ACTIVITY OF CALENDULA OFFICINALIS FLOWER EXTRACT USING FENTON REACTION

*Priyanka Pandey, Wasim Raja and S. Karmakar

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Abstract

Calendula officinalis is a good source of natural antioxidants, particularly phenolic substances, and other compounds that trap harmful free radicals, It is also known as “marigold” and has been a subject of several chemical and pharmacological studies. It is used in traditional medicine, especially for wound healing, jaundice, blood purification, and as an antispasmodic. There has been growing interest in the health benefits associated with natural compounds and have been demonstrated with the emphasis on antioxidants. The present study focused on the antioxidant activity of Calendula officinalis flower Extract in vitro conditions. The total phenolics content of leaf as determined by Fenton reaction and was found to be good antioxidant activity as different dose concentrations. The Reducing power of extract was carried out with ascorbic acid as a standard reducing agent. In this plant Calendula officinalis flower Extract there was a remarkable concentration dependent free radical scavenging and reducing power was exhibited. These findings demonstrated that Calendula officinalis flower extract possess free radical and hydroxyl radical scavenging activity as well as antioxidant activity in vitro. In conclusion the present study indicates that Calendula officinalis flower Extract may be a potential source of natural antioxidant.

Keywords: Antioxidant activity, Fenton Reaction, Hydroxyl radical, ascorbic acid, Calendula officinalis, Flower Extract, TBARS.


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