Abstract

ANTI-MICROBIAL STUDIES OF AQUEOUS, METHANOLIC AND SAPONINS EXTRACT OF SEEDS OF TRIGONELLA FOENUMGRAECUM ON HUMAN VAGINAL PATHOGENS CAUSING UTI INFECTION

*Swati Rawat and Raghvendra Dubey

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Abstract

The aqueous, methanolic and saponin extracts of Trigonella foenum- Graecum seeds were screened for antimicrobial activities against some human vaginal pathogens Staphylococcus aureus, Pseudomonas aeruginosa, streptococcus facecalis, klebsiella pneumoniae, Escherichia coli, Enterobacter faecalis, Enterobacter faecium and Proteus mirabilis isolated from patient samples. Extracts were found to produce significant inhibition against all the pathogens. Saponin extract were observed to be more active than methanolic and aqueous fraction. The minimum inhibitory concentration lies in the range from 08μg/ml to 48μg/ml. The percentage of relative inhibition zone diameter (% RIZD) observed to be in the range 41.73%-85.21%. Extracts are found to be more active against klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa and Enterobacter faecalis strains.

Keywords: Trigonella foenum- Graecum, Human Vaginal Pathogens, Saponin.