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Abstract

NEW SMARTPHONE BASED COLORIMETRIC METHOD DEVELOPMENT AND VALIDATION OF SODIUM DODECYL SULPHATE IN BULK AND DOSAGE FORM

Keyur Parmar* and Rajashree Mashru

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Abstract

Colorimetry is a method for determining the concentration of coloured substances in a solution. The intensity of colour is directly proportional to the concentration of compound being measured. Smartphone based colorimetry have grown in popularity as analytical instruments due to their low cost and ability to collect, store and process data all in one device. In smartphone colorimetry, the camera on the phone serves as a detector. The colorimetric method based on a smartphone and the UV method were both based on the detection of colour intensity as concentration increased. The developed method uses methylene blue as a dye to form a complex with SLS in a 1:1 ratio. The developed method exhibits good linearity ranging from 1-2μg/ml. With increasing the concentrations of API, the colour intensity increases. Samples were detected at 653 nm using UV-visible spectroscopy. Photometrix PRO software was used to analyse using mobile phone. A comparative study was done Using statistical tool i.e two paired test on both procedures conventional colorimetry and novel mobile phone analysis, the results show that both are equally significant.

Keywords: UV spectrophotometry, Smartphone based colorimetry, Photometrix PRO, RGB Histogram.


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