Abstract

EXTRACTION AND IDENTIFICATION OF PHYTOCHEMICAL ACTIVE CONSTITUENTS IN LEAVES OF CATHARANTHUS ROSEOUS, CARICA PAPAYA AND PIPER BETEL BY USING TLC METHOD

*V. Sai Muneeswari, Karthick S., D. Akanksha, G. Sushma, Sk. Abdul Hasheem,

Abstract

In the present research article attempt has been made to explain the extraction and identification of phytochemical active constituents in plants by using the TLC method. The selected plants having a rare pharmacological activity. These plants are extracted by using a simple method of extraction i.e. maceration and decoction method. Phytochemical screening of total ethanolic and chloroform extracts showed the presence of alkaloids, flavonoids, tannins, saponins and glycosides. A TLC is less time consuming, low cost and can be performed with less complicated technique it has a wide application in pharmaceutical analysis. TLC method can be used as a preliminary analytical method prior to HPLC. TLC can be used to monitor the progress of reaction, identify compound present in a given substances. TLC is also used to separate the identical compounds in a mixture. TLC studies constituted different coloured phytochemicals compounds with different rf values. This can be further investigated for the isolation and identification of the active bio chemical compound of medicinal utility in a cost effective way.

Keywords: analytical methods, maceration, decoction, phytochemicals, TLC method.