Abstract

EXTRACTION, PHYTOCHEMICAL SCREENING AND HEPATOPROTECTIVE ACTIVITY OF DACTYLORHIZA HATAGIREA ROOT EXTRACT

Indrakumar Sanodiya*, Dr. Praveen Kumar Jain, Rahim Khan, Dr. Brajesh Ku. Arjariya, Dr. Mohd. Jalaluddin and Ankit Mehra

Abstract

Natural products are playing a vital role in health care for decades. Often different sources of natural products, plants have been a source of chemical substance, which serves as drugs in their own right or key ingredients in formulation containing synthetic drugs. Present Investigation deals with hepatoprotective effect of hydroalcoholic extract of roots of Dactylorhiza hatagirea. The plant will be collected from local area of Bhopal, India. The authenticity of the plant was confirmed by botanist. The preliminary phytochemical analysis of the plant extracts will be performed using the standard protocol. Evaluate the hepatoprotective activity of hydroalcoholic extract using Paracetamol induced hepatotoxicity. The present study revealed a significant increase in the marker enzymes like AST, ALT, ALP and serum bilirubin levels, on exposure to Paracetamol, indicating considerable hepatocellular injury. Oral administration of Hydroalcoholic extracts of Dactylorhiza hatagirea (Root) at two different dose levels attenuated the increased levels of the marker enzymes produced by Paracetamol and caused a subsequent recovery towards normalization almost like that of standard silymarin treatment. The results showed that the Hydro-alcoholic extracts of Dactylorhiza hatagirea (Root) at different dose levels offer hepatoprotection.

Keywords: natural products, Dactylorhiza hatagirea (Root), Hydro-alcoholic extracts, hepatotoxicity.