Abstract

EVALUATION OF PROTECTIVE EFFECT OF ATORVASTATIN IN COMBINATION WITH ZINC SULPHATE ON ACETIC ACID INDUCED ULCERATIVE COLITIS IN RATS

Amit Sonkar* and Punam Sachdeva

Abstract

The purpose of this study was to evaluate the protective effect of Atorvastatin in combination with zinc sulphate on acetic acid induced Ulcerative Colitis in rats. 24 Sprague Dawley rats were divided into four groups containing six rats each. Group I (Model Control Group) was administered 2 ml of 4% acetic acid. Group II (Standard treatment Group) was administered 5 - ASA (Aminosalicylic acid) (30 mg/kg/day) p. o.. Groups III and IV (Drug Treatment Groups) were administered Atorvastatin (20 mg/kg/day) p. o. and Atorvastatin (20 mg/kg/day) + Zinc Sulphate (60 mg/kg/day) p.o., respectively. Colitis was induced by intracolonic administration of 2ml of 4% acetic acid on 7th day of start of the study. All drugs were administered daily for 6 days before to 7 days after intracolonic administration of 2 ml 4% Acetic acid. Various parameters, including Disease Activity Index score, Macroscopic damage score, Oxidative stress parameters and histopathological changes were evaluated to determine the severity of the colitis induced.

Keywords: Ulcerative Colitis, Acetic acid, Atorvastatin, Zinc Sulphate, Oxidative Stress.