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Abstract

SYNERGISTIC ACTIVITY OF ARK ON AMOXICILLIN AGAINST GRAM POSITIVE AND GRAM NEGATIVE BACTERIAL STRAINS

Sindhoora D.*, Vijesh V., Yashaswini, Nisha, Karunakar Hegde and A. R. Shabaraya

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Abstract

Objective: To evaluate the synergistic activity of Ark on Amoxicillin against Gram positive and Gram negative bacterial strains. Method: The antimicrobial activity was conducted by Agar plate well diffusion method by using Mueller Hinton Agar (MHA) media. 0.2 ml of Gram positive bacteria (S.aureus) and Gram negative bacteria (E.coli) were uniformly spread onto the each agar plate. 8 mm bores each at an equal distance from one another on the medium were made in which standard, test and combination drugs were incorporated. Four sets of agar plate each for Gram positive and Gram negative was prepared. Set 1 is control and Set 2 consists of Amoxicillin (20μg). Set 3 consists of Ark (20μl) and Set 4 consists of combination of Amoxicillin and Ark (20μg+20μl). Antimicrobial activity was measured by measuring the zone of inhibition. Results: The combination of Ark and Amoxicillin showed significant increase in antibacterial activity by increasing the zone of inhibition. The result obtained was compared with the other groups. Conclusion: With the findings of the present study it can be concluded that the Amoxicillin (20μg) and Ark (20μl) combination possessed significant increase in antimicrobial activity.

Keywords: Ark, Amoxicillin, Muller Hinton Agar (MHA) Media,anti-microbial activity.


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