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Abstract

FORMULATION AND EVALUATION OF HERBAL ANTIBACTERIAL GEL CONTAINING METHANOLIC EXTRACT OF ANNONA SQUAMOSA LEAVES

Roli Parihar* and Sandeep Jain

Abstract

The antibacterial gel was formulated and characterized anti containing methanolic extrract of Annona squamosa leaves and to evaluate the gel for various physical parameters, antibacterial activity and stability. The viscosity of 1.0 % w/v carbopol containing gel base was found to be 2129 cp with a spreading diameter of 57 mm while the viscosity of a 3.0 % w/v carbopol containing gel was obtained to be 3469 cp with a spreading diameter of 36 mm. The herbal gel formulations were prepared using 0.5, 1.0, 1.5 and 2.0 % w/w of Annona squamosa leaf extract and glycerin was incorporated in to formulations to provide moisturizing properties to the gel. All the herbal gel formulations except G4 were having a uniform consistency and no odor. G4 on the other hand was found to have a slight odor and grittiness. The pH of all the formulations was between 5.2-5.3 and was in the range of the pH of the skin (5.0-5.5). The formulations exhibited good spreadability and viscosity. The antibacterial activity of the herbal gel formulations was evaluated by cup and plate method using Staphylococcus aureus microbial culture. The zone of inhibition exhibited by G3 was the highest (19 mm) making it at the most effective antibacterial formulation. The accelerated stability study of G3 revealed no change in any of the physical properties of the gel formulation.

Keywords: Herbal, Antibacterial, Gel, Annona Squamosa, Carbopol.


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