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Dr. Dhrubo Jyoti Sen
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Abstract

A COMPARATIVE STUDY OF PHYTOCHEMICAL AND MEDHYA KARMA (MEMORY ENHANCING ACTIVITY) OF NATURALLY GROWN VACHA (ACORUS CALAMUS LINN.) AND VACHA (ACORUS CALAMUS LINN.) PROPAGATED BY TISSUE CULTURE IN MICE – A PROTOCOL

Prashant A. Pendor*, Sangita Jogadand and Nilima Wadanerwa

Abstract

Background: Some medicinal plant has special requirement for successful cultivation. The plant species are increasingly rare due to over exploitation by various local tribes of medicine and pharmaceutical industries. Number of this medicinal plant is now facing red listed category. Many medicinal plants species at high risk. Plant tissue culture technology has been widely used for large number of plant multiplication, plant propagation and treated diseases. In short period of time, nowadays many Medicinal drugs are not available. So, to overcome this problem we should accept different method by which drug may be available for treated disease. In such a scenario like tissue culture, artificial propagation will be use. In comparison to demand there is less cultivation of Vacha (Acorus calamus Linn.) This is making the plant endangered and research on MEA as tissue culture in Vacha (Acorus calamus Linn.) are knee of interest as these drugs they are capable of improving memory according to Ayurveda. Thus, comparative study of Vacha (Acorus calamus Linn.) collected from natural habitat and grown from different part of plants through tissue culture was not done as well the study of these two-drug analysis. Aims and Objectives: Comparative study of Phytochemicals and Medhya Karma (Memory Enhance activity) of naturally grown Vacha (Acorus calamus Linn.) and Vacha (Acorus calmus Linn.) propagated by Tissue Culture in Mice. Methodology: Procedure in this examination 2 sample of Vacha (acorus calamus Linn.) i.e., naturally grown and tissue cultured Vacha (Acorus calamus Linn.) will be separated and it will be divided into 36 mice in 6 group (6 mice in each). This sample will be carried out to evaluate Memory Enhance Activity by two methods Morris water maze and elevated plus maze. Expected Results: Result will be noted during physical and chemical analysis and during the study. Conclusion: Conclusion will be drawn on the basis of observation at the end of study.

Keywords: Vacha (Acorus calamus Linn.), Tissue culture technique, Memory enhancing activity.


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