Abstract

HPTLC FINGERPRINTING STUDY OF HYDROALCOHOLIC EXTRA CT FOR STEROIDS FROM Ailanthus excelsa (Roxb.) LEAVES

Amita Tilak*, Sudhir Singh Gangwar and Mudit Kumar

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Abstract

Objective: The present study was conducted to identify the steroids from Hydroalcohol extract of medicinally and economically useful leaves of Ailanthus excels (Roxb.) using High Performance Thin Layer Chromatography (HPTLC) technique. Methods: Preliminary phytochemical screening was done and HPTLC studies were carried out. CAMAG HPTLC system equipped with Linomat V applicator (S witzerland). Densitometric scanning was performed with Camag TLC scanner IV in the reflectance absorbance mode at 540 nm and operated by Win CATS software (1.4.6 Camag) with the help of tungstant lamp. Results: HPTLC finger printing of steroids of Hydroalcohol extract of leaves shows absence of steroids as no purple and maroon bands are seen after derivatisation with Anisaldehyde sulphuric acid. Conclusions: With the results of HPTLC analysis we have concluded the absence of steroids in the extract as no purple and maroon bands are seen after derivatisation with Anisaldehyde sulphuric acid.

Keywords: Ailanthus excelsa (Roxb.) leaves, steroids, HPTLC Fingerprinting.