Abstract

FORMULATION AND EVALUATION OF CURCUMIN AND ANDROGRAPHOLIDE PHYTOSOMES FOR THEIR POTENT ACTIVITIES

*Isha Wakde, Dr. Dinesh M. Biyani and Dr. Milind Umekar

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Abstract

The objective of this study was to develop phytosomes of curcumin in combination with andographolide for their potent therapeutic activity. The phytosomal complex was prepared by using solvent evaporation method. The independent variables amount of lipid (Phospholipon 90H) and amount of layer stagnant (cholesterol) were studied at three levels and arranged in a 32 factorial design to study the influence on the response variables- % entrapment efficiency (%EE) and In vitro drug release (% release) at 12 hours. The %EE and % drug release at 12 h for the 9 batches (F1 to F9) showed a variation of 28.87-54.45 % (curcumin), 60- 78.3 % (andrographolide), 34.76 – 49.85 % (curcumin) and 54.62 – 75.075 % (andrographolide) respectively. Optimized formulation showed particle size of 0.412μm, % EE of 54.45% (curcumin); 78.3% (andrographolide) and 49.85 % (curcumin); 75.075 % (andrographolide) % In vitro drug release after 12 h. The phytosomal complex was found to show cell cytotoxicity at a concentration of 103.12 μg/ml. Fourier Transformation Infrared Spectroscopy (FTIR) showed the absence of new bands for phytosome indicating no interaction between drugs and lipid and being only dissolved in it. Electron microscope of transmission techniques indicated cuboidal form of prepared phytosome in dry state (due to reduced interfacial tension) with smooth surface with size approximately around 200 μm.

Keywords: curcumin, andrographolide, phytosomes, anticancer effect, combination of curcumin and andrographolide phytosomes.