WJPR Citation New

  All Since 2011
 Citation  2903  2393
 h-index  27  24
 i10-index  68  60

Login

Best Paper Awards

World Journal of Pharmaceutical Research (WJPR) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.
Best Paper Award :
Dr. Dhrubo Jyoti Sen
Download Article: Click Here

Search

Track Your Article

Abstract

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF RP-HPLC FOR THE DETERMINATION OF AZELNIDIPINE IN BULK AND PHARMACEUTICAL DOSAGE FORM

Girindra Lekhi*, Chandanam Sreedhar, T. Srinivasa Rao, Harsha K. Tripathy, Deepthi D. Kini and Manju S. V.

Abstract

The new RP-HPLC method for the estimation of Azelnidipine was found out by using different chromatographic parameters. Chromatography was performed by gradient reverse phase separation using a Water’s XBridge C18 column of particle size 5μ 250×4.6mm. The separations were achieved at the UV detection at 255nm using the mobile phase of Potassium dihydrogen phosphate and orthophosphoric acid (buffer): Methanol (60:40v/v). Flow rate was 1ml/min and the injection volume was set at 20 μl with 10 mins of runtime. The retention time was observed at 4.49 mins. The method was validated by using various validation parameters like accuracy, precision, linearity, limit of detection (LOD), limit of quantification (LOQ). These results showed the method could find practical application as a quality control tool for analysis of the drug in its pharmaceutical dosage forms in quality control laboratories. The standard curve was linear over a working range of 5-30 μg/ml and gave an average correlation factor of 0.9984 for Azelnidipine. The limit of detection and the limit of quantitation were found to be 1.38μg/ml μg/ml and 4.17μg/ml respectively. The method showed good recoveries and relative standard deviations of intra and inter day assay less than 2. This method can be easily and conveniently used for routine analysis of Azelnidipine in bulk and tablet dosage forms.

Keywords: Azelnidipine, RP-HPLC, Accuracy, Precision, Linearity, LOD, LOQ.


[Full Text Article]

Call for Paper

World Journal of Pharmaceutical Research (WJPR)
Read More

Email & SMS Alert

World Journal of Pharmaceutical Research (WJPR)
Read More

Article Statistics

World Journal of Pharmaceutical Research (WJPR)
Read More

Online Submission

World Journal of Pharmaceutical Research (WJPR)
Read More