Abstract

PHYTOCHEMICAL SCREENING AND EVALUATION OF ANTIBACTERIAL ACTIVITY OF CLEOME BRACHYCARPA EXTRACT

N. Bhojak* Amita and Seema Vyas

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Abstract

The present investigations have been designed with a motive to develop green method to extract different potential phytochemicals found in aerial part of Cleome brachycarpa and to evaluate their biological activity. The extracts of Cleome brachycarpa were prepared by using Soxhlet extraction protocol and by a developed green method. Different solvents i.e. water, alcohol, petroleum ether and micellar medium were used successively to extract different photochemicals. Preliminary phytochemical screening of extract showed the presence of flavonoids, glycosides, triterpenoids, phenolic compounds. The antibacterial activities of extracts were evaluated by disc diffusion method. For Biological evolution screening of extracts have been carried out against Escherichia coli, Bascillus subtilis and Staphylococcus aureus.

Keywords: Phytochemical screening, antibacterial activity, Cleome brachycarpa, extract.