PRELIMINARY STUDY FOR THE EFFECT OF DIONYSIA AUCHERI METHANOLIC EXTRACT ON THE VIABILITY OF SOME CELL LINES
Khulood W Alsamarrae*, Zainab Yaseen and Saman Abdulrahman Ahmad
Abstract
The purpose of present study is a cytotoxic investigation for Dionysia
aucheri methanolic extract on different cell lines. Methods used for
that: HPLC fractionation; the fractions proceeded according to
protocol of the pharmacology department, university of Malaya,
Kuala Lumpur, Malaysia, and the largest purified fraction was
used for MTT test. Four types of cell lines (Normal human
hepatic cell line (WRL), Human lung adenocarcinoma cell line
(A549), Human hepatic carcinoma cell line (HEPG2) and Human
prostate carcinoma cell line (PC-3)) were treated with six two
fold concentrations (100,50,25,12.5, 6.25 and 3.1285) μg/ml, at 24
hour interval. Results showed that the extract affected all types of cell line in a dose
dependent manner. The most important conclusion in this study was the significant effect of
the all D.aucheri extract concentrations on the viability of human hepatic carcinoma (HepG-
2) cell line, while little cytotoxic effect of the methanolic extract was on the human prostate
carcinoma (PC-3) cells which might give the plant an importance to be a promise phyto
protective agent. This made an imported demand to search and investigates the plant active
components that affect even normal cells.
Keywords: (cytotoxic investigation, Dionysia aucheri, methanolic extract, HPLC, WRL cell line, HEPG-2 cell line, A549 cell line, PC-3 cell line, Phytoprotective agent).
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