Abstract

PRELIMINARY STUDY FOR THE EFFECT OF DIONYSIA AUCHERI METHANOLIC EXTRACT ON THE VIABILITY OF SOME CELL LINES

Khulood W Alsamarrae*, Zainab Yaseen and Saman Abdulrahman Ahmad

Abstract

The purpose of present study is a cytotoxic investigation for Dionysia aucheri methanolic extract on different cell lines. Methods used for that: HPLC fractionation; the fractions proceeded according to protocol of the pharmacology department, university of Malaya, Kuala Lumpur, Malaysia, and the largest purified fraction was used for MTT test. Four types of cell lines (Normal human hepatic cell line (WRL), Human lung adenocarcinoma cell line (A549), Human hepatic carcinoma cell line (HEPG2) and Human prostate carcinoma cell line (PC-3)) were treated with six two fold concentrations (100,50,25,12.5, 6.25 and 3.1285) μg/ml, at 24 hour interval. Results showed that the extract affected all types of cell line in a dose dependent manner. The most important conclusion in this study was the significant effect of the all D.aucheri extract concentrations on the viability of human hepatic carcinoma (HepG- 2) cell line, while little cytotoxic effect of the methanolic extract was on the human prostate carcinoma (PC-3) cells which might give the plant an importance to be a promise phyto protective agent. This made an imported demand to search and investigates the plant active components that affect even normal cells.

Keywords: (cytotoxic investigation, Dionysia aucheri, methanolic extract, HPLC, WRL cell line, HEPG-2 cell line, A549 cell line, PC-3 cell line, Phytoprotective agent).