Abstract

PHARMACOGNOSTIC AND PHYTOCHEMICAL INVESTIGATION, IN-VITRO ANTI-OXIDANT AND ANTI-INFLAMMATORY STUDIES OF METHANOLIC EXTRACT OF CENTRATHERUM PUNCTATUM CASS. LEAVES FORMULATED IN CREAM

Shilpashree V. K., Saima Parveen*, Sayyed Sarowsh, Moona Thasleema and Ravikumar

Abstract

Centratherum punctatum Cass. also called lark daisy is a medicinal plant belonging to the family Asteraceae. It is a low maintenance perennial herb of 45-60cm in height grown in tropical areas. According to previous article leaves of this plant showed various medicinal properties such as anti-inflammatory, anti-microbial, anti-oxidant, and anti-proliferative activities. The present investigation is to formulate a cream containing methanolic extract of Centratherum punctatum leaves. The formulated cream was evaluated for various parameters and analysed for anti-oxidant and anti-inflammatory activities. Total phenolic content was estimated by FC [Folin-Ciocalteu] method. The methanolic extract of Centratherum punctatum showed good amount of phenolic content which might be responsible for its anti-oxidant and anti-inflammatory activities. Anti-oxidant properties were detected by FRAP [Ferric Reducing Anti-oxidant Power] assay and anti-inflammatory was investigated by protein denaturation method. The methanolic extract of Centratherum punctatum leaves when formulated to cream showed effective results and exhibits anti-oxidant and anti-inflammatory properties.

Keywords: Centratherum punctatum, phenolic content, anti-inflammatory, anti-oxidant, FRAP, protein denaturation.